Guides/Bacteriostatic Water
Essentials

Bacteriostatic Water

Bacteriostatic Water for Peptides: The Complete Guide

Last updated: March 2026

Sequence
0.9% benzyl alcohol in sterile water
Origin
Pharmaceutical-grade preparation
Available From
7 suppliers
Forms
3 types

Key Benefits

1
Inhibits bacterial growth via 0.9% benzyl alcohol, enabling safe multi-dose vial use
2
Essential for reconstituting lyophilized (freeze-dried) peptides before administration
3
Allows multi-dose use from a single vial โ€” unlike single-use sterile water
4
USP-grade formulation with a well-established safety profile in research settings

How It Works

Bacteriostatic water (BAC water) is sterile water containing 0.9% benzyl alcohol as a preservative. The benzyl alcohol inhibits bacterial growth, making it safe to use the same vial multiple times over up to 28 days after opening. This is critical for peptide research because most peptide vials contain multiple doses. When BAC water is added to a lyophilized peptide, it reconstitutes the powder into a solution that can be accurately dosed. Without the bacteriostatic agent, each vial puncture risks introducing bacteria that could contaminate the solution. BAC water vs. sterile water vs. bacteriostatic sodium chloride: Use BAC water as the default for peptide reconstitution. Use sterile water only for single-use applications or when benzyl alcohol sensitivity is a concern. Use bacteriostatic sodium chloride (0.9% NaCl with benzyl alcohol) when isotonicity is required. For step-by-step reconstitution instructions, use our reconstitution calculator at /tools/reconstitution-calculator.

Research Highlights

๐Ÿ“„Benzyl alcohol at 0.9% concentration effectively prevents bacterial proliferation for up to 28 days (USP <797> guidelines)
๐Ÿ“„Standard reconstitution solvent recommended in peptide research protocols worldwide
๐Ÿ“„Step-by-step reconstitution: clean vial tops with alcohol swabs, inject BAC water slowly against the vial wall, gently swirl (never shake) until fully dissolved
๐Ÿ“„Common mistakes: using tap or distilled water (non-sterile), shaking the vial (denatures peptides), reusing BAC water beyond 28 days, storing at room temperature after opening
๐Ÿ“„Storage: unopened vials stable at room temperature (15-25ยฐC). After first puncture, refrigerate at 2-8ยฐC and discard after 28 days
๐Ÿ“„Bacteriostatic water is available from peptide suppliers, compounding pharmacies, and medical supply stores โ€” always verify USP grade and 0.9% benzyl alcohol concentration

Available Forms

  • Pre-filled vials (10 mL, 20 mL, 30 mL most common)
  • Multi-pack options for frequent research use
  • USP-grade from compounding pharmacies

Research Dosing

1-2 mL per peptide vial depending on desired concentration. Use our reconstitution calculator at /tools/reconstitution-calculator to determine the exact volume for your peptide and target dose.

โš ๏ธ For research reference only. Not medical advice.

L
Licensed Peptides
lyophilized powder ยท ยท โ€”
65
Fair
$7.95
โ€”
L
Licensed Peptides
lyophilized powder ยท ยท โ€”
65
Fair
$7.95
โ€”
B
Beyond Peptides
lyophilized powder ยท ยท โ€”
80
Good
$9.91
โ€”
B
Beyond Peptides
lyophilized powder ยท ยท โ€”
80
Good
$9.91
โ€”
I
Ion Peptide
ยท ยท โ€”
55
Fair
$700.00
โ€”
R
Ruo Bio
lyophilized powder ยท ยท โ€”
55
Fair
Contact supplier
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